Sterilization is the highest 
          level of disinfection. 
          Because tattoo and piercing needles penetrate into the body they are required to be sterile. To maintain sterility 
          everything that touches that needle is a potential source of 
			pathogens. 
          
          
          Sterility is a probability, not an absolute. 
          Though "Sterilization" is meant to convey an
            absolute (the destruction or inactivation of all 
          microorganisms - either something is sterile or it is not) 
             
                 A. It cannot be known whether all 
			microorganisms
            have been killed. 
                      1. We may not 
			be aware of the existence of some. 
                      2. We may not
            actually be culturing for all microorganisms. 
			 
                 B. We cannot prove the existence of a
            negative absolute. 
			 
            Therefore the approach is to employ a "process"
            definition. 
             
			
				Sterilization is the process by which living organisms are
            removed or killed to the extent that they are no longer detectable
            in standard culture media in which they had previously
            proliferated.   (Block, 4th Edition, Dis. Ster. Pres.) 
			 
			
          
          Both the process and the methods used to test are equally important. 
			In actuality it is a probability and not an absolute. 
			
          
           
          
          Sterility measurement is expressed as a probability. 
          
          Sterility is considered achieved when it reaches what 
          is called a "log of minus 6 of reduction" rather than as an
            absolute and is called the "Sterility Assurance Level" (SAL) which
            can be quantitatively (in numbers) expressed.  This means: One million microbes is the basis for the calculations, which is 
			why prior cleaning is necessary. Too many microbes on the items, 
			more than one million, would require a greater processing time or 
			temperature.. 
			
           
            
            Each negative log represents a 90% reduction of microbes. 
            Imagine a glass full of water. Assume for a moment the water
            represents the microbes and it equals one million microbes (estimated to be on your
            product). This is a "Log of 6" ( a log of 6 equals 1 million 
			microbes).   
			
          If we empty 90% of the water it leaves 10% of the 
			water.  Our original one million  microbes - our water,  minus 90% is
            100,000 microbes left. Because we are reducing we go down the number 
			of reductions by 90% logs, as it were,
            and now have a "log of 5" or, now only 10,000 microbes.  90% 
			of the 100,000 have been
            killed or inactivated).   
			
          Repeating our process again, we empty 90% of what is left over in the
            glass. This is a "log of 4" (10,000-10% microbes left over 
			from the first step, minus 90% leaves 1,000 
			microbes 
			
          106 1,000,000 microbes 
			105 1,000,000 minus 90% = 100,000 
			104 100,000 minus 90% = 10,000 
			103 10,000 minus 90% = 1,000 
			102 1,000 minus 90% = 100 
			101 100 minus 90% = 10 
			100 10 minus 90% = 1 
			10-1 1 minus 90% of 1? - can't have 10% of one microbe
			 
       but we can think of it as 1 microbe among 
			10 items. = -1 
			
          If we do this a total of 6 times we have a
            "log of 0", which would be one microbe left.  If we
            now continue and want to empty 90%
            of what's left (our 1 microbe) of course we can't empty 90% of just 
			one microbe.  
			 
            But we can think of it as a probability: In other words 
            If we had one item we would have one microbe on that item.   
			If we had 10 items we can say we might have one microbe among ten items 
			(a 90% reuction), 
			
          .  This level is 10 -1, Ten to the 
			minus one meaning one microbe per 10 items. A
            log of reduction to 10-2 therefore means one microbe per 100 items, etc. A log of 
			10-6 means  
			one microbe
            might survive on a million items  
			presuming that the original
            microbial load was one million microbes or less. This is what is
            called a  Sterility Assurance Level (SAL) 10-6 =  One 
			microbe per million items. That's it. 
            This is the level that your sterilizer must produce to be acceptable 
			as "sterile,"  a log of 10-6.  
			 
            The level of disinfection-sterilization varies according to the 
			appropriate use of the item. 
			 
			Topically applied "sterile" products, 
			a log of 10-3 is considered suitable. 
			 
			Implantables require a SAL of 10-6 Log. 
			 
            Normal saturated steam processing produces one log of reduction
            (90% "kill") every
            1 to 2 minutes at 15 Lbs. psi. and 250°F. 
			 
            "Sterility" under the conditions of saturated steam can be reached 
			in about 12 to 15 minutes and an additional 5 minutes is considered 
			precautionary for "overkill." 
			A holding time of 15 to 20 
			minutes at temperature and pressure is considered adequate 
			"overkill."  
  
			It would be un-reasonable to extend this 
			time or leave the sterilizer run longer than 20 minutes 
			without known and good reason. In tattoo and piercing non-hospital environment. 
			You 
			can certainly give your clients the greatest confidence that the 
			products you are using are sterile. 
			 
			The importance of cleaning 
			is to reduce the
            original microbial load so that you can be sure of this SAL of
            10-6.  If you started with 10 billion microbes ( a
            quantity of 1010) you can see that
            to reach a probability of 1
            in a million items (10-6) will require
            a longer cycle per minute time or higher
            temperatures. (Sterilization Technology) 
			 
            Items should be cleaned, dried and placed into sterilization
            pouches. Though air is a source of microbes to possibly contaminate
            items after washing and before bagging, even hospital air has been
            measured to only an estimated load of 128 microbes per cubic meter, so
            air is not of great concern unless you live in areas that have
            a heavy soil particle air count. Soils are the predominant source
            of spores. 
            Overkill is a term referring to the additional time a
            sterilizer operates beyond the time necessary to reach the kill
            level of sterilization.  The common reference is the bacterial
            spore G. Stearolthermphilus which is used for biological
            monitoring of the sterilization process, which is usually in your
            spore test  that you send for testing.   
            Normal
            sterilization of the BI can usually be achieved in 6 minutes with the rest of
            the time considered "overkill."  A holding time of 15
            to 20 minutes at temperature and pressure is considered adequate
            "overkill."  Tests may show adverse indicators if the 
			test is held at either a much higher temperature or much longer 
			dwell times.  
			Higher temperatre and longer dwell may be evaluated as a failed 
			test. Flash 
			Sterilizatio: fast, higher temperature, is not 
			designed for routine sterilization.  |